منابع مشابه
A test of a counting model for chiasma interference.
According to the model of FOSS, LANDE, STAHL and STEINBERG, chiasma interference is a reflection of the requirement for crossovers to be separated by an organism-specific number of potential conversion events without associated crossovers. This model predicts that tetrads with close double crossovers should be enriched for conversion events that themselves are not associated with crossing over....
متن کاملTranscriptomic landscape of prophase I sunflower male meiocytes
Meiosis is a form of specialized cell division that generates gametes, allowing recombination of alleles and halving the chromosome number. Arabidopsis and maize are the plant models that have been most extensively studied to determine the genes involved in meiosis. Here we present an RNA-seq study in which gene expression in male meiocytes isolated during prophase I was compared to that in som...
متن کاملSequencing-based large-scale genomics approaches with small numbers of isolated maize meiocytes
High-throughput sequencing has become the large-scale approach of choice to study global gene expression and the distribution of specific chromatin marks and features. However, the limited availability of large amounts of purified cells made it very challenging to apply sequencing-based techniques in plant meiosis research in the past. In this paper, we describe a method to isolate meiocytes fr...
متن کاملChiasma F'requency and Distribution in a Maize Family Segregating for Kio and Trisomy
Chiasma frequency was determined from total chiasma counts, and the distribution of these exchanges was determined by the ratio of proximal to distal chiasmata. No effect of trisomy IO could be demonstrated. Confirmation was obtained of earlier work showing more proximal and fewer distal chiasmata in K10 plants than in controls. However, diakinesis data failed to confirm the ability of KIO to i...
متن کاملCytomixis doesn’t induce obvious changes in chromatin modifications and programmed cell death in tobacco male meiocytes
Cytomixis is a poorly studied process of nuclear migration between plant cells. It is so far unknown what drives cytomixis and what is the functional state of the chromatin migrating between cells. Using immunostaining, we have analyzed the distribution of posttranslational histone modifications (methylation, acetylation, and phosphorylation) that reflect the functional state of chromatin in th...
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2020
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.3604